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KMID : 1234420120400040310
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Korean Journal of Microbiololgy and Biotechnology
2012 Volume.40 No. 4 p.310 ~ p.318
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Lactococcus lactis Culture Methods for the Enhanced Depression of Inducers in Atopic Diseases
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Jo Yu-Ran
Kang Sang-Mo
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Abstract
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We conducted a screening and checked the cultivation methods of lactic acid bacteria, which have anti-atopic dermatitis functions, by determining the lactic acid bacteria's immune enhancement by FACS, and antimicrobial activity against Staphylococcus aureus. Increased CD4+/CD25+/foxp3+ in T cells resulting in immune enhancement effects were observed in decreasing significance in Lactobacillus plantarum, Lactococcus lactis subsp. lactis (Lc. lactis). Antimicrobial activity against S. aureus was noted in decreasing significance in Lc. Lactis and Lb. plantarum. The antimicrobial activity of Lb. plantarum culture with medium containing Lc. lactis culture broth was not enhanced, but the antimicrobial activity of Lc. lactis cultured in a medium containing Lb. plantarum culture broth was enhanced. As the optimal method caltivation of Lc. lactis in a medium containing 10% of heat-killed Lb. plantarum culture broth was chosen. By this method, the antibacterial activity of the pure Lc. lactis culture increased sharply at the end of the log phase, while a restraint effect on the growth of S. aureus increased 1.29 times.
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KEYWORD
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Lactobacillus plantarum, Lactococcus lactis subsp. lactis, immune enhancement, antimicrobial activity, Staphylococcus aureus, heat-killed
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